RBS

Part:BBa_K4392000:Design

Designed by: Vikram Venugopal   Group: iGEM22_IISER-Pune-India   (2022-09-30)


Ribosome binding site associated with the T7 system from the T7 bacteriophage


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 16
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 16
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 16
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Since the part was already a part of the pHis17_parM_1 backbone we used for our RF cloning experiment, there were no extraordinary considerations needed to be taken from our part.


Source

Originally sourced from the T7 bacteriophage, we have sourced it from the pHis17_parM_1 plasmid, the backbone of our restriction-free cloning.

References